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dapi  (MedChemExpress)


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    Structured Review

    MedChemExpress dapi
    Dapi, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vari+fluor+555+phalloidin/Vari+Fluor+555-Phalloidin/bio_rxiv__64898__2026__04__09__717383-166-59-54
    Average 94 stars, based on 2 article reviews
    dapi - by Bioz Stars, 2026-09
    94/100 stars

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    Incubation:

    Article Title: FOXA1 preserves cell polarity and restrains lysosome biogenesis in non-small cell lung adenocarcinoma
    Article Snippet: For immunofluorescence, cells were fixed with 4% paraformaldehyde for 15 min and permeabilized with 0.5% Triton X-100 in PBS for 5 min. After blocking with 3% BSA for 1 h at room temperature, the cells were washed three times in TBS-T (15 min per each wash) and incubated overnight with primary antibodies against lamp1 (1:100, CST 15665S), P63 (1:100, CST 39692S), TFE3 (1:100, Proteintech 14480-1-AP), and CDH1 (1:1000, CST 3195S). .. Following primary antibodies incubation, the cells were then washed three times in TBS-T (5 min per each wash) and incubated for 1 h in a dark humidity chamber with secondary antibodies Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (1:200, ab150077) or Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555), along with Vari Fluor 555-Phalloidin (1:200, MedChemExpress, HY-D1816-300T) and 0.5 mg/mL DAPI. ..

    Staining:

    Article Title: 3D printing of recombinant collagen/chitosan methacrylate/nanoclay hydrogels loaded with Kartogenin nanoparticles for cartilage regeneration
    Article Snippet: .. Scaffolds implanted with hBMSCs were stained using Vari Fluor 555-Phalloidin (MCE, USA) and 4′,6-diamidino-2-phenylindole (Solarbio, China) to assess the scaffold attachment effect on cells. ..



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